Product Description |
Recombinant Human ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Glu480. |
Product Background |
Intercellular adhesion molecule-1 (ICAM-1; CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
ICAM-1/CD54 |
Synonyms |
ICAM-1; BB2; CD54 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln28-Glu480 |
Molecular Weight |
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ICAM-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human ICAM-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ICAM-1 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. |
|
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Human ICAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human ICAM-1 is greater than 95% as determined by SEC-HPLC. |
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