Human IGF1R/CD221 Protein (IGF-HM41R)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IGF1R, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Glu31-Asn932

Catalog: IGF-HM41R

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Product Description	Recombinant Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Asn932.
Product Category	Recombinant Protein

Target	IGF1R/CD221
Synonyms	CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216
Gene/Protein ID	P08069
Species	Human
Exact Sequence	Glu31-Asn932
Molecular Weight	The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa(alpha subunit) and 52-55 kDa(beta subunit) based on Tris-Bis PAGE result.
Activity	Immobilized Human IGF1R, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IGF1R, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA (QC Test).
	Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA.
	The purity of Human IGF1R is greater than 95% as determined by SEC-HPLC.
	Human IGF1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.