Product Description |
Recombinant Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Glu31-Asn932. |
Product Background |
The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target. |
Product Category |
Recombinant Protein |
Target |
IGF1R/CD221 |
Synonyms |
CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu31-Asn932 |
Molecular Weight |
The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IGF1R, His Tag at 0.2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Immobilized Human IGF1R, His Tag at 0.2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 10.4 ng/ml determined by ELISA. |
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Immobilized Human IGF1R, His Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 1.25 ug/ml determined by ELISA (QC Test). |
|
|
Human IGF1R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IGF1R is greater than 95% as determined by SEC-HPLC. |
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