Human IL-10 R alpha Protein (IL1-HM4RA)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: His22-Asn235

Catalog: IL1-HM4RA

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Product Description Recombinant Human IL-10 R alpha Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains His22-Asn235.
Product Category Recombinant Protein
Target IL-10R alpha
Synonyms IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha
Gene/Protein ID Q13651
Species Human
Exact Sequence His22-Asn235
Molecular Weight The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 47.4ng/ml determined by ELISA.
The purity of Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-10 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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