Name: Human IL-17A&F Protein (ILF-HM118)
Brand: KactusBio
SKU: ILF-HM118-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Catalog #ILF-HM118

Size

100UG

500UG

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Product Description	Recombinant Human IL-17A&F Protein is expressed from HEK293 with His tag at the C-Terminus of IL-17F.It contains Gly24-Ala155(IL-17A) and Arg31-Gln163(IL-17F).
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-17A&F
Synonyms	IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
Accession	Q16552-1(IL-17A)&Q96PD4(IL-17F)
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight	The protein has a predicted MW of 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test).
	Immobilized Human IL-17A&F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
	Human IL-17A&F on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%
	The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC.