Product Description |
Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213. |
Product Background |
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-2 R alpha/CD25 |
Synonyms |
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu22-Cys213 |
Molecular Weight |
The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). |
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Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. |
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Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. |
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