Human IL-2 R alpha/CD25 Protein (CD5-HM425)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu22-Cys213
Catalog #CD5-HM425
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Product Description
 Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
Product Background
 Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category
 Recombinant Protein
Target
 IL-2 R alpha/CD25
Synonyms
 CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu22-Cys213
Molecular Weight
 The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA.
Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

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