Name: Non-biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425)
Brand: KactusBio
SKU: CD5-HM425-100UG
Price: 330.0 USD
Availability: InStock

Non-biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425)

Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).

Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.

Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu22-Cys213

Catalog #CD5-HM425

Size

100UG

500UG

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Product Description	Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
Product Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-2 R alpha/CD25
Synonyms	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Accession	P01589
Species	Human
Biotinylated	no
Amino Acid Range	Glu22-Cys213
Molecular Weight	The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Immobilized Human IL-2 R alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
	Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

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