Name: Human IL-27 Protein (IL2-HM127)
Brand: KactusBio
SKU: IL2-HM127-100UG
Price: 346.5 USD
Availability: InStock

Express System: CHO
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)

Catalog #IL2-HM127

Size

100UG

500UG

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Product Description	Recombinant Human IL-27 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A).
Product Background	Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-27
Synonyms	Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28;
Accession	Q14213(IL-27B)&Q8NEV9(IL-27A)
Species	Human
Biotinylated	no
Amino Acid Range	Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)
Molecular Weight	The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	CHO
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA (QC Test).
	Human IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.