Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein (ITG-HM2V3)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGAV&ITGB3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail

Catalog: ITG-HM2V3

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Product Description Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail.
Product Category Recombinant Protein
Target Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer
Gene/Protein ID P06756-1(ITGAV)&P05106-1(ITGB3)
Species Human
Exact Sequence Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail
Molecular Weight The protein has a predicted MW of 131.90 kDa (ITGAV)&101.79 kDa (ITGB3). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAV)&105-130 kDa (ITGB3) based on Tris-Bis PAGE result.
Activity Immobilized Human ITGAV&ITGB3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ITGAV&ITGB3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
The purity of Human ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC.
Human ITGAV&ITGB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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