Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein (ITG-HM4V5)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ITGAV&ITGB5, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 73.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe31-Val992(ITGAV)acidic tail & Gly24-Asn719(ITGB5)basic tail

Catalog: ITG-HM4V5

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Product Description Recombinant Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe31-Val992(ITGAV) acidic tail&Gly24-Asn719(ITGB5) basic tail.
Product Category Recombinant Protein
Target Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer
Synonyms Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5
Gene/Protein ID P06756-1(ITGAV)&P18084(ITGB5)
Species Human
Exact Sequence Phe31-Val992(ITGAV)acidic tail & Gly24-Asn719(ITGB5)basic tail
Molecular Weight The protein has a predicted MW of 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ITGAV&ITGB5, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 73.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ITGAV&ITGB5, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 73.9ng/ml determined by ELISA.
The purity of Human ITGAV&ITGB5 is greater than 95% as determined by SEC-HPLC.
Human ITGAV&ITGB5 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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