Name: Non-biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein (ITG-HM4V5)
Brand: KactusBio
SKU: ITG-HM4V5-100UG
Price: 412.5 USD
Availability: InStock

Non-biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein (ITG-HM4V5)

Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.

Human ITGAV&ITGB5-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Loaded Anti-alpha V Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human ITGAV&ITGB5, His-Avi Tag with an affinity constant of 57.4 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail

Catalog #ITG-HM4V5

Size

100UG

500UG

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Product Description	Recombinant Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Gly24-Asn719(ITGB5) basic tail.
Product Background	Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection)- Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer
Synonyms	αVβ5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V β 5; β5
Accession	P06756-1(ITGAV)&P18084(ITGB5)
Species	Human
Biotinylated	no
Amino Acid Range	Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail
Molecular Weight	The protein has a predicted MW of 114 kDa (ITGAV) & 81.4 kDa (ITGB5). Due to glycosylation,the protein migrates to 85-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
	Human ITGAV&ITGB5-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Loaded Anti-alpha V Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human ITGAV&ITGB5, His-Avi Tag with an affinity constant of 57.4 nM as determined in BLI assay (Gator® Prime).

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