ELISA sigmoidal binding curve of Human Latent TGF-beta 3 His-Tag protein with Human TGF-beta RII mFc-Tag receptor, plotting mean OD450 absorbance versus log receptor concentration (µg/ml).
Human Latent TGF beta 3/TGFB3 Protein (TGF-HM103)
Human Latent TGF beta 3/TGFB3 Protein (TGF-HM103)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Latent TGF beta 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu24-Ser412
Catalog #TGF-HM103
Regular price $445.50
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Product Description

Recombinant Human Latent TGF beta 3/TGFB3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu24-Ser412.

Product Background

Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
Latent TGF beta 3/TGFB3
Synonyms
TGFB3; ARVD; TGF-beta3; TGF-β; TGFβ3; TGFβ; TGF-beta 3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu24-Ser412
Molecular Weight
The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Latent TGF beta 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Latent TGF beta 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). Immobilized Human Latent TGF beta 3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Human Latent TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Latent TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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