Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly24-His458

Catalog: LIL-HM4B1

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Product Description	Recombinant Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-His458.
Product Background	LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Product Category	Recombinant Protein

Target	LILRB1/CD85j/ILT2
Synonyms	ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Gene/Protein ID	Q8NHL6-1
Species	Human
Exact Sequence	Gly24-His458
Molecular Weight	The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 72-78 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LILRB1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LILRB1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
	Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 23ng/ml.
	The purity of-Human LILRB1 is greater than 95% as determined by SEC-HPLC.
	Human LILRB1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.