Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1)
Catalog: LIL-HM4B1
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| Product Description | Recombinant Human LILRB1/CD85j/ILT2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly24-His458. |
| Product Category | Recombinant Protein |
| Target | LILRB1/CD85j/ILT2 |
| Synonyms | ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
| Gene/Protein ID | Q8NHL6-1 |
| Species | Human |
| Exact Sequence | Gly24-His458 |
| Molecular Weight | The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 72-78 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human LILRB1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human LILRB1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. |
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Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 23ng/ml. |
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The purity of Human LILRB1 is greater than 95% as determined by SEC-HPLC. |
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Human LILRB1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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