Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly24-His458

Catalog: LIL-HM4B1

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Product Description Recombinant Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-His458.

Product Background

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.

Product Category

Recombinant Protein

Target

LILRB1/CD85j/ILT2
Synonyms ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession

Species

Human

Amino Acid Range

Gly24-His458
Molecular Weight The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 72-78 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LILRB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LILRB1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 23ng/ml.
The purity of-Human LILRB1 is greater than 95% as determined by SEC-HPLC.
Human LILRB1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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