Name: Human LILRB2/CD85d/ILT4 Domain1&2 Protein (LIL-HM12D)
Brand: KactusBio
SKU: LIL-HM12D-100UG
Price: 270.0 USD
Availability: InStock

Human LILRB2/CD85d/ILT4 Domain1&2 Protein (LIL-HM12D)

Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).

Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).

Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43ug/ml.

Human LILRB2 Domain 1&2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of-Human LILRB2 Domain 1&2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test). The affinity constant of 6.5uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln22-Val229

Catalog #LIL-HM12D

Size

100UG

500UG

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Product Description	Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val229.
Product Background	The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB2/CD85d/ILT4 Domain1&2
Synonyms	CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Accession	Q8N423-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln22-Val229
Molecular Weight	The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test). The affinity constant of 6.5uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).
	Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).
	Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43ug/ml.
	Human LILRB2 Domain 1&2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of-Human LILRB2 Domain 1&2 is greater than 95% as determined by SEC-HPLC.