Human LILRB2/CD85d/ILT4 Domain1&2 Protein (LIL-HM12D)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human LILRB2 Domain1&2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. The affinity constant of 6.5μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln22-Val229

Catalog: LIL-HM12D

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Product Description Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Gln22-Val229.
Product Category Recombinant Protein
Target LILRB2/CD85d/ILT4 Domain1&2
Synonyms CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Gene/Protein ID Q8N423-1
Species Human
Exact Sequence Gln22-Val229
Molecular Weight The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LILRB2 Domain1&2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. The affinity constant of 6.5μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5μM as determined in a SPR assay (Biacore T200).
Immobilized Human LILRB2 Domain1&2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43μg/ml.
The purity of Human LILRB2 Domain 1&2 is greater than 95% as determined by SEC-HPLC.
Human LILRB2 Domain 1&2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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