Name: Human LILRB2/CD85d/ILT4 Domain1&2 Protein (LIL-HM12D)
Brand: KactusBio
SKU: LIL-HM12D-100UG
Price: 297.0 USD
Availability: InStock

Human LILRB2/CD85d/ILT4 Domain1&2 Protein (LIL-HM12D)

Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).

Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).

Human LILRB2 Domain 1&2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43ug/ml.

The purity of-Human LILRB2 Domain 1&2 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 6.5uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln22-Val229

Catalog #LIL-HM12D

Size

100UG

500UG

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Product Description	Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val229.
Product Background	The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	LILRB2/CD85d/ILT4
Synonyms	CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Accession	AAL36991.1
Species	Human
Biotinylated	no
Amino Acid Range	Gln22-Val229
Molecular Weight	The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 6.5uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).
	Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).
	Human LILRB2 Domain 1&2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43ug/ml.
	The purity of-Human LILRB2 Domain 1&2 is greater than 90% as determined by SEC-HPLC.

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