Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LILRB3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly24-Glu443

Catalog: LIL-HM4B3

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Product Description Recombinant Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.

Product Background

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.

Product Category

Recombinant Protein

Target

LILRB3/CD85a/ILT5
Synonyms CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9
Accession

Species

Human

Amino Acid Range

Gly24-Glu443
Molecular Weight The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LILRB3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LILRB3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA.
The purity of Human LILRB3 is greater than 95% as determined by SEC-HPLC.
Human LILRB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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