Product Description |
Recombinant Human LRP-5-Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu644-Gln1263. |
Product Background |
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis. |
Product Category |
Recombinant Protein |
Target |
LRP-5 |
Synonyms |
LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu644-Gln1263 |
Molecular Weight |
The protein has a predicted MW of 96.51 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human LRP-5, hFc Tag captured on CM5 Chip via Protein A can bind Human DKK1, His Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test). |
|
|
Human LRP-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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