Product Description |
Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Leu385. |
Product Background |
MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1]. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
MANSC1 |
Synonyms |
MANSC1; LOH12CR3; 9130403P13Rik |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln27-Leu385 |
Molecular Weight |
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human MANSC1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. |
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Human MANSC1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human MANSC1 is greater than 95% as determined by SEC-HPLC. |
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