Human MCP-1/CCL2 Protein (MCP-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Gln24-Thr99

Catalog: MCP-HM401

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Product Description Recombinant Human MCP-1/CCL2 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Thr99.

Product Background

 Monocyte chemotactic protein-1 (MCP-1) (also referred to as chemokine (C-C motif) ligand 2 (CCL2) is expressed by mainly inflammatory cells and endothelial cells. MCP-1 has been reported to play an important role in the pathogenesis of atherosclerosis and considerable evidence supports that the monocyte containing MCPs and macrophage influences the growth of other cell types within the atherosclerotic lesion. This review will focus on the general structure features of MCP-1 and its role in atherosclerosis.

Product Category

 Recombinant Protein

Target

 MCP-1/CCL2
Synonyms C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; HSMCR30; MCP1; SCYA2; SMC-CF; GDCF-2

Gene/Protein ID

 P13500

Species

 Human

Exact Sequence

 Gln24-Thr99
Molecular Weight The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human MCP-1 is greater than 95% as determined by SEC-HPLC.
Human MCP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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