Non-biotinylated Human MCP-1/CCL2 Protein (MCP-HM401)
Non-biotinylated Human MCP-1/CCL2 Protein (MCP-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln24-Thr99
Catalog #MCP-HM401
Regular price $297.00
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Product Description

Recombinant Human MCP-1/CCL2 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Thr99.

Product Background

Monocyte chemotactic protein-1 (MCP-1) (also referred to as chemokine (C-C motif) ligand 2 (CCL2) is expressed by mainly inflammatory cells and endothelial cells. MCP-1 has been reported to play an important role in the pathogenesis of atherosclerosis and considerable evidence supports that the monocyte containing MCPs and macrophage influences the growth of other cell types within the atherosclerotic lesion. This review will focus on the general structure features of MCP-1 and its role in atherosclerosis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
MCP-1/CCL2
Synonyms
C-C motif chemokine 2; CCL2; HC11; MCAF; MCP-1; HSMCR30; MCP1; SCYA2; SMC-CF; GDCF-2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln24-Thr99
Molecular Weight
The protein has a predicted MW of 11.5 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human MCP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human MCP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MCP-1 is greater than 95% as determined by SEC-HPLC. The purity of Human MCP-1 is greater than 95% as determined by SEC-HPLC.
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