Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Summary
Product Description |
Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat). |
Product Background |
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Specifications/Details
Protein |
GDF-8 |
Synonyms |
Myostatin; GDF8; Mstn; GDF-8; Myostatin |
Accession |
|
Species |
Human/Mouse/Rat |
Biotinylated |
no |
Amino Acid Range |
Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat) |
Molecular Weight |
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Please refer to the Certificate of Analysis for detailed instructions. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). |
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Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. |
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Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA. |
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Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. |
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Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test). |
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