- Express System: HEK293
- Product Tag: No Tag
- Purity: >95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. The affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Asp267-Ser375
Product Description |
Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375. |
Product Background |
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain. |
Product Category |
Recombinant Protein |
Target |
GDF-8 |
Accession |
|
Species |
Human/Mouse/Rat |
Biotinylated |
no |
Amino Acid Range |
Asp267-Ser375 |
Molecular Weight |
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. The affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4 mM HCl. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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