Human/Mouse/Rat GDF-8 Protein (GDF-HM008)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp267-Ser375

Catalog: GDF-HM008

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375.
Product Background	Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category	Recombinant Protein

Target	GDF-8
Accession	O14793
Species	Human/Mouse/Rat
Amino Acid Range	Asp267-Ser375
Molecular Weight	The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 4mM HCl.
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
	Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is < 30 ng/ml (QC Test).
	Human/Mouse/Rat GDF-8 on Tris-Bis PAGE under reduced (R) condition. The purity is greater than 95%.