Name: Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
Brand: KactusBio
SKU: GDF-HM008-100UG
Price: 660.0 USD
Availability: InStock

Human/Mouse/Rat GDF-8 Protein (GDF-HM008)

Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).

Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).

Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).

Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.

Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.

Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.

Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
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Catalog #GDF-HM008

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Summary

Product Description	Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat).
Product Background	Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Specifications/Details

Protein	GDF-8
Synonyms	Myostatin; GDF8; Mstn; GDF-8; Myostatin
Accession	O14793(Human) / O08689(Mouse) / O35312(Rat)
Species	Human/Mouse/Rat
Biotinylated	no
Amino Acid Range	Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
Molecular Weight	The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 4 mM HCL Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
	Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
	Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).
	Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
	Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.
	Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.
	Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
	Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).