Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. The affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp267-Ser375
Catalog #GDF-HM008
Regular price $600.00
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Product Description
Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375.
Product Background
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Product Category
Recombinant Protein
Target
GDF-8
Accession
Species
Human/Mouse/Rat
Biotinylated
no
Amino Acid Range
Asp267-Ser375
Molecular Weight
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. The affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
No Tag
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4 mM HCl.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA.
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test).
Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.
Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).

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