Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
Human/Mouse/Rat GDF-8 Protein (GDF-HM008)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
Catalog #GDF-HM008
Regular price $660.00
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Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Summary

Product Description

Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat).

Product Background

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human/Mouse/Rat GDF-8 Protein (GDF-HM008) Specifications/Details

Protein
GDF-8
Synonyms
Myostatin; GDF8; Mstn; GDF-8; Myostatin
Accession
Species
Human/Mouse/Rat
Biotinylated
no
Amino Acid Range
Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
Molecular Weight
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Product Tag
No Tag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Please refer to the Certificate of Analysis for detailed instructions.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200). Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test). Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 ug/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA. Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA.
Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA. Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.
Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test). Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).
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