Product Description |
Recombinant Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Leu20-Gly718. |
Product Background |
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
NCAM-1/CD56 |
Synonyms |
N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Gly718 |
Molecular Weight |
The protein has a predicted MW of 78.48 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NCAM-1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human NCAM-1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA. |
|
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Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NCAM-1 is greater than 95% as determined by SEC-HPLC. |
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