Name: Non-biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401)
Brand: KactusBio
SKU: NEC-HM401-100UG
Price: 264.0 USD
Availability: InStock

Non-biotinylated Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401)

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).

Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).

Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test).

Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA.

Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test). The affinity constant of 28.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln31-Thr334

Catalog #NEC-HM401

Size

100UG

500UG

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Product Description	Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334.
Product Background	Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	Nectin-1/PVRL1/CD111
Synonyms	CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR
Accession	Q15223-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln31-Thr334
Molecular Weight	The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test). The affinity constant of 28.13 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).
	Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).
	Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test).
	Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA.
	Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC.

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