Name: Human OX40 Ligand/TNFSF4 Protein (OXL-HM240)
Brand: KactusBio
SKU: OXL-HM240-100UG
Price: 297.0 USD
Availability: InStock

Human OX40 Ligand/TNFSF4 Protein (OXL-HM240)

Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).

Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).

Human OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human OX40 Ligand is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln51-Leu183

Catalog #OXL-HM240

Size

100UG

500UG

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Product Description	Recombinant Human OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Gln51-Leu183.
Product Background	Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	OX40 Ligand/TNFSF4
Synonyms	OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34
Accession	P23510-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln51-Leu183
Molecular Weight	The protein has a predicted MW of 40.95 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
	Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
	Human OX40 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human OX40 Ligand is greater than 95% as determined by SEC-HPLC.

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