Product Description |
Recombinant Human OX40 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu29-Ala216. |
Product Background |
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
OX40/TNFRSF4/CD134 |
Synonyms |
CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu29-Ala216 |
Molecular Weight |
The protein has a predicted MW of 46.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA (QC Test). |
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Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human OX40, hFc Tag on ProA-Biosensor, can bind Human OX40 Ligand (Trimer), His Tag with an affinity constant of 0.16 nM as determined in BLI assay (Gator® Prime). |
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The purity of Human OX40 is greater than 95% as determined by SEC-HPLC. |
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