Name: Human PD-1/PDCD1 Protein (PD1-HM301)
Brand: KactusBio
SKU: PD1-HM301-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu25-Gln167

Catalog #PD1-HM301

Size

100UG

500UG

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Product Description	Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.
Product Background	Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Accession	Q15116-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Gln167
Molecular Weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-PD-1 Ab., hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Human PD-1 on Bis-Tris PAGE under reduced condition.The purity is greater than 95%.
	The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.