Name: Non-biotinylated Human PDGF R beta/CD140b Protein (PGF-HM4RB)
Brand: KactusBio
SKU: PGF-HM4RB-100UG
Price: 247.5 USD
Availability: InStock

Non-biotinylated Human PDGF R beta/CD140b Protein (PGF-HM4RB)

Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.

Human PDGF R beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human PDGF R beta is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu33-Phe530

Catalog #PGF-HM4RB

Size

100UG

500UG

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Product Description	Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu33-Phe530.
Product Background	PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	PDGF R beta/CD140b
Synonyms	JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-β; PDGF R β
Accession	P09619
Species	Human
Biotinylated	no
Amino Acid Range	Leu33-Phe530
Molecular Weight	The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PDGF R beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.
	Human PDGF R beta on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human PDGF R beta is greater than 95% as determined by SEC-HPLC.

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