ELISA binding curve of human Periostin His-tag protein showing OD450 absorbance response to increasing concentrations of anti-Periostin hFc-tagged antibody.
Human Periostin/OSF-2 Protein (OSF-HM102)
Human Periostin/OSF-2 Protein (OSF-HM102)
Human Periostin/OSF-2 Protein (OSF-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn22-Gln836
Catalog #OSF-HM102
Regular price $363.00
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Product Description

Recombinant Human Periostin/OSF-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn22-Gln836.

Product Background

Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
Periostin/OSF-2
Synonyms
Periostin; PN; OSF-2; POSTN; OSF2; Fasciclin I-like; PDLPOSTN; PNRP11-412K4.1; TRIF52
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asn22-Gln836
Molecular Weight
The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized Human Periostin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Human Periostin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Periostin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Periostin is greater than 95% as determined by SEC-HPLC. The purity of Human Periostin is greater than 95% as determined by SEC-HPLC.
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