Human PGF Protein (PGF-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu19-Arg221

Catalog: PGF-HM201

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Product Description	Recombinant Human PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221.
Product Background	Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
Product Category	Recombinant Protein

Target	PGF
Synonyms	PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
Gene/Protein ID	P49763-1
Species	Human
Exact Sequence	Leu19-Arg221
Molecular Weight	The protein has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
	Immobilized Human PGF, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test).
	The purity of Human PGF is greater than 95% as determined by SEC-HPLC.
	Human PGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.