Product Description |
Recombinant Human PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221. |
Product Background |
Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients. |
Product Category |
Recombinant Protein |
Target |
PGF |
Synonyms |
PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu19-Arg221 |
Molecular Weight |
The protein has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. |
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Immobilized Human PGF, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test). |
|
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Human PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human PGF is greater than 95% as determined by SEC-HPLC. |
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