Name: Human PGF Protein (PGF-HM201)
Brand: KactusBio
SKU: PGF-HM201-100UG
Price: 210.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu19-Arg221

Catalog #PGF-HM201

Size

100UG

500UG

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Product Description	Recombinant Human PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221.
Product Background	Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
Product Category	Recombinant Protein / Antibody discovery

Protein	PGF
Synonyms	PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
Accession	P49763-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu19-Arg221
Molecular Weight	The protein has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
	Immobilized Human PGF, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test).
	Human PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PGF is greater than 95% as determined by SEC-HPLC.