Name: Human PGF Protein (PGF-HM201)
Brand: KactusBio
SKU: PGF-HM201-100UG
Price: 231.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PGF, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu19-Arg221

Catalog #PGF-HM201

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Arg221.
Product Background	Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	PGF
Synonyms	PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
Accession	P49763-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu19-Arg221
Molecular Weight	The protein has a predicted MW of 48.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PGF, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human PGF, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody, hFc Tag with the EC50 of 34.1ng/ml determined by ELISA (QC Test).
	Immobilized Human PGF, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
	Human PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PGF is greater than 95% as determined by SEC-HPLC.