Human PKC iota Protein (PKC-HB101)
  • Express System: Baculovirus-Insect Cells
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Met10-Val596
Catalog #PKC-HB101
Regular price $300.00
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Product Description
Recombinant Human PKC iota Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met10-Val596.
Product Background
PKClambda/iota belongs to the third group of the PKC family, atypical PKC (aPKC), together with PKCzeta based on its sequence divergence from conventional and novel PKCs observed not only in the N-terminal regulatory domain but also in the kinase domain. Although one of the most distinct features of aPKC is its single, unrepeated cysteine-rich domain, recent studies have revealed that the N-terminal regulatory domain has additional aPKC-specific structural motifs involved in various protein-protein interactions, which are important for the regulation and the subcellular targeting of aPKC.
Product Category
Recombinant Protein
Target
PKC iota
Synonyms
nPKC-iota; PRKCI; DXS1179E;
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met10-Val596
Molecular Weight
The protein has a predicted MW of 68.78 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
Baculovirus-Insect Cells
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PKC iota on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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