Human PKC iota Protein (PKC-HB101)

  • Express System: Baculovirus-Insect Cells
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Met10-Val596

Catalog: PKC-HB101

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Product Description Recombinant Human PKC iota Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met10-Val596.

Product Background

 PKClambda/iota belongs to the third group of the PKC family, atypical PKC (aPKC), together with PKCzeta based on its sequence divergence from conventional and novel PKCs observed not only in the N-terminal regulatory domain but also in the kinase domain. Although one of the most distinct features of aPKC is its single, unrepeated cysteine-rich domain, recent studies have revealed that the N-terminal regulatory domain has additional aPKC-specific structural motifs involved in various protein-protein interactions, which are important for the regulation and the subcellular targeting of aPKC.

Product Category

 Recombinant Protein

Target

 PKC iota

Gene/Protein ID

 P41743

Species

 Human

Exact Sequence

 Met10-Val596
Molecular Weight The protein has a predicted MW of 68.78 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 Baculovirus-Insect Cells
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PKC iota on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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