Name: Human RANKL/TNFSF11/CD254 Protein (RKL-HM101)
Brand: KactusBio
SKU: RKL-HM101-100UG
Price: 346.5 USD
Availability: InStock

Human RANKL/TNFSF11/CD254 Protein (RKL-HM101)

Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.

Human Rankl on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly63-Asp244

Catalog #RKL-HM101

Size

100UG

500UG

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Product Description	Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gly63-Asp244.
Product Background	Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	RANKL/TNFSF11/CD254
Synonyms	ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Accession	O14788-2
Species	Human
Biotinylated	no
Amino Acid Range	Gly63-Asp244
Molecular Weight	The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 35-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human RANKL, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
	Human Rankl on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.