Name: Human RGM-C Protein (RGM-HM10C)
Brand: KactusBio
SKU: RGM-HM10C-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln36-Asp400

Catalog #RGM-HM10C

Size

100UG

500UG

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Product Description	Recombinant Human RGM-C Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln36-Asp400.
Product Background	RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	RGM-C
Synonyms	Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
Accession	Q6ZVN8-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln36-Asp400
Molecular Weight	The protein has a predicted MW of 40 kDa (mature), 26 kDa (C-terminus peptide) and 14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa, 30-40 kDa, 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human RGM-C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Human RGM-C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-RGM-C Ab., hFc-Avi Tag on ProA-Biosensor can bind Human RGM-C, His Tag with an affinity constant of 4.59 nM as determined in BLI assay (Gator® Prime).
	The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC.