Human S100A8 Protein (SA8-HE101) Summary
Product Description |
Recombinant Human S100A8 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Glu93. |
Product Background |
S100A8 and S100A9 (also known as MRP8 and MRP14, respectively) are Ca2 binding proteins belonging to the S100 family. They often exist in the form of heterodimer, while homodimer exists very little because of the stability. S100A8/A9 is constitutively expressed in neutrophils and monocytes as a Ca2 sensor, participating in cytoskeleton rearrangement and arachidonic acid metabolism. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human S100A8 Protein (SA8-HE101) Specifications/Details
Protein |
S100A8 |
Synonyms |
Calgranulin-A; CFAG; MRP-8; p8; CAGA; S100A8; 60B8AG; CGLA; CP-10; NIF; L1Ag; MA387; MIF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Glu93 |
Molecular Weight |
The protein has a predicted MW of 11.7 kDa same as Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human S100A8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human S100A8 is greater than 95% as determined by SEC-HPLC. |
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