Name: Non-biotinylated Human Siglec-6/CD327 Protein (SIG-HM406)
Brand: KactusBio
SKU: SIG-HM406-100UG
Price: 346.5 USD
Availability: InStock

Non-biotinylated Human Siglec-6/CD327 Protein (SIG-HM406)

Anti-Siglec-6-Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Siglec-6, His Tag with an affinity constant of 0.16 nM as determined in SPR assay (Biacore T200).

Immobilized Human Siglec-6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).

Human Siglec-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-Siglec-6-Antibody,hFc Tag on ProA-Biosensor, can bind Human Siglec-6, His Tag with an affinity constant of 0.06 nM as determined in BLI assay (Gator).

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human Siglec-6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln27-Val331

Catalog #SIG-HM406

Size

100UG

500UG

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Product Description	Recombinant Human Siglec-6/CD327 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln27-Val331.
Product Background	Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Siglec-6
Synonyms	CD327; CD33 antigen-like 1; CD33L1; CDw327; OB-BP1; Siglec-6; SIGLEC6; CD33L; OBBP1
Accession	O43699-3
Species	Human
Biotinylated	no
Amino Acid Range	Gln27-Val331
Molecular Weight	The protein has a predicted MW of 36.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.16 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-Siglec-6-Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Siglec-6, His Tag with an affinity constant of 0.16 nM as determined in SPR assay (Biacore T200).
	Immobilized Human Siglec-6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-6 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human Siglec-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-Siglec-6-Antibody,hFc Tag on ProA-Biosensor, can bind Human Siglec-6, His Tag with an affinity constant of 0.06 nM as determined in BLI assay (Gator).

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