Product Description |
Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213. |
Product Background |
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
Product Category |
Recombinant Protein |
Target |
SOST/Sclerostin |
Synonyms |
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Tyr213 |
Molecular Weight |
The protein has a predicted MW of 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SOST, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human SOST, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. |
|
|
Human SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Related Products
Recently viewed
