Human Tim-3/HAVCR2 Protein (TIM-HM131)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser22-Arg200

Catalog: TIM-HM131

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Product Description	Recombinant Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Arg200.
Product Background	Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.
Product Category	Recombinant Protein

Target	Tim-3/HAVCR2
Synonyms	CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3
Gene/Protein ID	Q8TDQ0-1
Species	Human
Exact Sequence	Ser22-Arg200
Molecular Weight	The protein has a predicted MW of 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
	The purity of Human TIM-3 is greater than 95% as determined by SEC-HPLC.
	Human TIM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.