Human Tim-3/HAVCR2 Protein (TIM-HM131)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TIM-3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser22-Arg200

Catalog: TIM-HM131

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Product Description Recombinant Human Tim-3/HAVCR2 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ser22-Arg200.
Product Category Recombinant Protein
Target Tim-3/HAVCR2
Synonyms CD366; TIM3; TIM-3; TIMD-3; TIM 3; FLJ14428; HAVCR2; KIM3
Gene/Protein ID Q8TDQ0-1
Species Human
Exact Sequence Ser22-Arg200
Molecular Weight The protein has a predicted MW of 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TIM-3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TIM-3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
The purity of Human TIM-3 is greater than 95% as determined by SEC-HPLC.
Human TIM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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