Human TIM-3/HAVCR2 Protein (TIM-HM131)

Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

Human TIM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human TIM-3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Amino Acid Range: Ser22-Arg200

Catalog #TIM-HM131

Price

$264⁰⁰

Size

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Human TIM-3/HAVCR2 Protein (TIM-HM131) Summary

Product Description

Recombinant Human TIM-3/HAVCR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Arg200.

Product Background

Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Human TIM-3/HAVCR2 Protein (TIM-HM131) Specifications/Details

Protein	TIM-3/HAVCR2
Synonyms	CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3;TIMD3
Accession	Q8TDQ0-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser22-Arg200
Molecular Weight	The protein has a predicted MW of 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
	Human TIM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TIM-3 is greater than 95% as determined by SEC-HPLC.