Human Tim-3/HAVCR2 Protein (TIM-HM131)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser22-Arg200

Catalog: TIM-HM131

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Product Description Recombinant Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Arg200.

Product Background

Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Product Category

Recombinant Protein

Target

Tim-3/HAVCR2
Synonyms CD366; TIM3; TIM-3; TIMD-3; TIM 3;-FLJ14428; HAVCR2; KIM3
Accession

Species

Human

Amino Acid Range

Ser22-Arg200
Molecular Weight The protein has a predicted MW of 20.7 kDa, Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TIM-3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TIM-3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
The purity of Human TIM-3 is greater than 95% as determined by SEC-HPLC.
Human TIM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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