Name: Human TRAIL/TNFSF10 Protein (TRL-HE001)
Brand: KactusBio
SKU: TRL-HE001-100UG
Price: 346.5 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val114-Gly281

Catalog #TRL-HE001

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human TRAIL/TNFSF10 Protein is expressed from E.coli without tag.It contains Val114-Gly281.
Product Background	Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	TRAIL/TNFSF10
Synonyms	Apo-2L; TRAIL; CD253; TNFSF10; Apo-2 ligand; APO2L; TL2
Accession	NP_003801.1
Species	Human
Biotinylated	no
Amino Acid Range	Val114-Gly281
Molecular Weight	The protein has a predicted MW of 19.6 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 300mM NaCl (pH 7.2). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TRAIL R3, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).
	Immobilized Human TRAIL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
	Human TRAIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC.