Human uPAR/PLAUR Protein (PAR-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Human PLAU, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu23-Gly305

Catalog: PAR-HM401

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Product Description Recombinant Human uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu23-Gly305.

Product Background

The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

Product Category

Recombinant Protein

Target

uPAR/PLAUR

Gene/Protein ID

Q03405-1

Species

Human

Exact Sequence

Leu23-Gly305
Molecular Weight The protein has a predicted MW of 34.36 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Human PLAU, His Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human uPAR, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Human PLAU, His Tag with the EC50 of 9.6ng/ml determined by ELISA.
The purity of Human uPAR is greater than 95% as determined by SEC-HPLC.
Human uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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