Human VEGF R2/KDR Protein (VGF-HM4R2)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF R2 at 0.5ug/ml (100ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala20-Glu764

Catalog: VGF-HM4R2

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Product Description	Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Glu764.
Product Background	Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Product Category	Recombinant Protein

Target	VEGF R2/KDR
Synonyms	CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Gene/Protein ID	P35968-1
Species	Human
Exact Sequence	Ala20-Glu764
Molecular Weight	The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human VEGF R2 at 0.5ug/ml (100ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human VEGF R2 at 0.5ug/ml (100ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
	The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
	Human VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.