Human VEGF R2/KDR Protein (VGF-HM4R2)
Human VEGF R2/KDR Protein (VGF-HM4R2)
Human VEGF R2/KDR Protein (VGF-HM4R2)
Human VEGF R2/KDR Protein (VGF-HM4R2)
Human VEGF R2/KDR Protein (VGF-HM4R2)
Human VEGF R2/KDR Protein (VGF-HM4R2)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human VEGF R2 at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-HM4R2
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Product Description
 Recombinant Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Ala20-Glu764.
Product Background
 Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Product Category
 Recombinant Protein
Target
 VEGF R2/KDR
Synonyms
 CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala20-Glu764
Molecular Weight
 The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF R2 at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF R2 at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-VEGFR2 Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA.
Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
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