Product Description |
Recombinant Human VIPR2 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Glu24-Val126. |
Product Background |
Effects of vasoactive intestinal peptide (VIP) on T cell migration are mediated by structurally distinct types I (VIPR1) and II (VIPR2) G protein-associated receptors. The two receptor types were proposed to transduce opposite effects on human T cells, since cytokine-induced chemotaxis of VIPR1-bearing HuT 78 human T cells, in contrast to T cells that express VIPR2, was inhibited by VIP. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
VIPR2 |
Synonyms |
VIP-R-2; VPAC2; PACAP-R-3; PACAP-R3; VIPR2; VIP2R |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu24-Val126 |
Molecular Weight |
The protein has a predicted MW of 37.35 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-mFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human VIPR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human VIPR2 is greater than 95% as determined by SEC-HPLC. |
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