Human Vitronectin Protein (VTN-HM101)
Human Vitronectin Protein (VTN-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp20-Leu478
Catalog #VTN-HM101
Regular price $45.00
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Product Description

Recombinant Human Vitronectin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Leu478.

Product Background

Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1.

Product Category

Recombinant Protein / Antibody discovery

Protein

Vitronectin

Synonyms

VN; S-protein; Serum-spreading factor; V75; VTN

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp20-Leu478

Molecular Weight

The protein has a predicted MW of 53.37 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA.
Human Vitronectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Vitronectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Vitronectin is greater than 95% as determined by SEC-HPLC. The purity of Human Vitronectin is greater than 95% as determined by SEC-HPLC.

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