Product Description |
Recombinant Human Vitronectin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Leu478. |
Product Background |
Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1. |
Product Category |
Recombinant Protein |
Target |
Vitronectin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp20-Leu478 |
Molecular Weight |
The protein has a predicted MW of 53.37 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. |
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|
Human Vitronectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Vitronectin is greater than 95% as determined by SEC-HPLC. |
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