Human Vitronectin Protein (VTN-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp20-Leu478

Catalog: VTN-HM101

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Product Description Recombinant Human Vitronectin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Leu478.

Product Background

Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1.

Product Category

Recombinant Protein

Target

Vitronectin
Accession

Species

Human

Amino Acid Range

Asp20-Leu478
Molecular Weight The protein has a predicted MW of 53.37 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA.
The purity of Human Vitronectin is greater than 95% as determined by SEC-HPLC.
Human Vitronectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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