- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA.
- Amino Acid Range: Asp20-Leu478
Human Vitronectin Protein (VTN-HM101) Summary
Product Description |
Recombinant Human Vitronectin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Leu478. |
Product Background |
Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Vitronectin Protein (VTN-HM101) Specifications/Details
Protein |
Vitronectin |
Synonyms |
VN; S-protein; Serum-spreading factor; V75; VTN |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp20-Leu478 |
Molecular Weight |
The protein has a predicted MW of 53.37 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Vitronectin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04ug/ml determined by ELISA. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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