Mouse CD180 Protein (CD1-MM180) Summary
Product Description |
Recombinant Mouse CD180 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr21-Ser626. |
Product Background |
CD180, a related member of the Toll-like receptor family, is lost or underexpressed at the plasma membrane in circulating cells of various B-cell lymphomas except marginal zone lymphomas (MZL).CD180 can be considered as a single positive robust marker of MZL and should be therefore included in flow cytometry panels for the diagnosis of mature B-cell neoplasms. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Mouse CD180 Protein (CD1-MM180) Specifications/Details
Protein |
CD180 |
Synonyms |
CD180 antigen;Lymphocyte antigen 78;Ly-78;CD180;Rp105;LY64 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr21-Ser626 |
Molecular Weight |
The protein has a predicted MW of 69.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse CD180 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse CD180 is greater than 95% as determined by SEC-HPLC. |
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