Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
Mouse CD27 Ligand/CD70 Protein (CD7-MM270)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser45-Pro195
Catalog #CD7-MM270
Regular price $297.00
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Product Description

Recombinant Mouse CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ser45-Pro195.

Product Background

CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD27 Ligand/CD70

Synonyms

CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ser45-Pro195

Molecular Weight

The protein has a predicted MW of 43.41 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 6.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. Immobilized Mouse CD27, mFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA.
Mouse CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD27 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Mouse CD27 Ligand is greater than 95% as determined by SEC-HPLC.

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