Product Description |
Recombinant Mouse M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Ser511. |
Product Background |
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
M-CSF R/CSF1R/CD115 |
Synonyms |
CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala20-Ser511 |
Molecular Weight |
The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 76-82 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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|
Mouse M-CSF R, His tag captured on CM5 Chip via Anti-His Antibody can bind Human M-CSF, No tag with an affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 1.69ug/ml determined by ELISA (QC Test). |
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Mouse M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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