Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein (MHC-MM452)
Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein (MHC-MM452)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
  • Amino Acid Range: His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide
Catalog #MHC-MM452
Regular price $310.00
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Product Description
Recombinant Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide.
Product Background
Qa-1b binds a peptide (AMAPRTLLL), referred to as Qdm (for Qa-1 determinant modifier), derived from the signal sequence of murine class Ia molecules. This peptide binds with high affinity and accounts for almost all of the peptides associated with this molecule. Human histocompatibility leukocyte antigen (HLA)-E, a homologue of Qa-1b, binds similar peptides derived from human class Ia molecules and interacts with CD94/NKG2 receptors on natural killer cells.
Product Category
Recombinant Protein
Target
Q1-1b&B2M&Qdm (AMAPRTLLL) Monomer
MHC Form
Monomer
Allele
Qa-1b
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
His23-Pro296(Qa-1b), Ile21-Met119(B2M) and AMAPRTLLL peptide
Molecular Weight
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Qa-1b&B2M&Qdm (AMAPRTLLL) Monomer is greater than 95% as determined by SEC-HPLC.

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