Name: Mouse R spondin 1/RSPO1 Protein (RS1-MM101)
Brand: KactusBio
SKU: RS1-MM101-100UG
Price: 412.5 USD
Availability: InStock

Mouse R spondin 1/RSPO1 Protein (RS1-MM101)

Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.

Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser21-Gln265

Catalog #RS1-MM101

Size

100UG

500UG

Order now, ships by:

[{"id":42618392477918,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"RS1-MM101-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Mouse R spondin 1\/RSPO1 Protein (RS1-MM101) - 100UG","public_title":"100UG","options":["100UG"],"price":41250,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584185762014,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"RS1-MM101-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Mouse R spondin 1\/RSPO1 Protein (RS1-MM101) - 500UG","public_title":"500UG","options":["500UG"],"price":137500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Gln265.
Product Background	The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	R spondin 1/RSPO1
Synonyms	R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
Accession	Q9Z132
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser21-Gln265
Molecular Weight	The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.
	Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

Product Description

Product Background

Product Category

Product Tag