Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr70-Asp316
Catalog #RKL-MM101
Regular price $346.50
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Product Description

Recombinant Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr70-Asp316.

Product Background

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
RANKL/TNFSF11/CD254
Synonyms
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Tyr70-Asp316
Molecular Weight
The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Mouse RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC. The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC.
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