Product Description |
Recombinant Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr70-Asp316. |
Product Background |
Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
RANKL/TNFSF11/CD254 |
Synonyms |
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Tyr70-Asp316 |
Molecular Weight |
The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test). |
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Mouse RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC. |
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