Product Description |
Recombinant Mouse Siglec-5/CD170 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr17-Leu439. |
Product Background |
Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Siglec-5/CD170 |
Synonyms |
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr17-Leu439 |
Molecular Weight |
The protein has a predicted MW of 46.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Mouse Siglec-5/CD170 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse Siglec-5/CD170 is greater than 95% as determined by SEC-HPLC. |
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