Name: Mouse TNFRSF12A/TWEAKR Protein (TNF-MM20A)
Brand: KactusBio
SKU: TNF-MM20A-100UG
Price: 231.0 USD
Availability: InStock

Mouse TNFRSF12A/TWEAKR Protein (TNF-MM20A)

Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).

Mouse TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse TNFRSF12A is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu28-Per80

Catalog #TNF-MM20A

Size

100UG

500UG

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Product Description	Recombinant Mouse TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Glu28-Per80.
Product Background	Thyroid cancer (TC) is the most well-known endocrine neoplasia as well as a common malignant tumor in the head and neck.TNFRSF12A expression may be a potential useful prognostic molecular biomarker of bad survival in thyroid cancer, in addition, PPAR signaling pathway, insulin signaling pathway, mTOR signaling pathway may be the key pathway controlled by TNFRSF12A in thyroid cancer. Further experimental ought to be performed to demonstrate the biologic effect of TNFRSF12A.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	TNFRSF12A/TWEAKR
Synonyms	FGF-inducible 14; Fgfrp2; TNFRSF12A; FN14; CD266; TWEAKR
Accession	Q9CR75
Species	Mouse
Biotinylated	no
Amino Acid Range	Glu28-Per80
Molecular Weight	The protein has a predicted MW of 32.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
	Mouse TNFRSF12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse TNFRSF12A is greater than 95% as determined by SEC-HPLC.

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