Product Description |
Recombinant Mouse TNFSF12/TWEAK Protein is expressed from HEK293 with Rabbit Fc tag at the N-terminus.It contains Ser46-His249. |
Product Background |
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
TNFSF12/TWEAK |
Synonyms |
TNFSF12; APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ser46-His249 |
Molecular Weight |
The protein has a predicted MW of 48.95 kDa. Due to furin cleavage and glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). The affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-Rabbit Fc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Mouse TNFSF12, Rabbit Fc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.12 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse TNFRSF12A, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF12, Rabbit Fc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). |
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Mouse TNFSF12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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